Paraffin Sectioning

Trim fixed tissues into appropriate size and shape and place in embedding cassettes.

Paraffin sectioning. Process for paraffin embedding schedule as follow. Safety microtome setup microtome blades trimming facing and roughing blocks techniques for consistent paraffin sections microtome maintenance common microtomy faults. The result of this procedure for mounting sections is to allow any section from a sample to be equated with its position in the sample tissue or relative to other sections in the series.

Place the blade in the holder ensure it is secure and set the clearance angle. Paraffin sectioning is the procedure of cutting thin slices of tissue that has been dehydrated and infiltrated with wax using specialized equipment. Paraffin processing of tissue where the best possible morphology is required animals should be anesthesized and subjected to cardiac perfusion with.

Paraffin sectioning and mounting protocol. In practice this protocol is much easier to follow than it is to explain. This tissue is then embedded in wax before being cut on a microtome.

This protocol describes a method for embedding tissues in paraffin blocks for sectioning. Fill a waterbath with ultrapure water and heat to 40 45 o c. For routine stains where perfusion is not required tissue is sectioned and drop fixed in a 10 formalin solution.

Section paraffin blocks at the desired thickness usually 4 5 µm on a microtome and float on a 40 c water bath. Sectioning chill paraffin embedded tissue blocks on ice before sectioning. Due to the slow rate of diffusion.

Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Paraffin sections are more physically stable and superior to frozen sections in maintaining tissue morphology with less damage. The clearance angle prevents.