Oct Sectioning

Embed the tissue completely in oct compound prior to cryostat sectioning.

Oct sectioning. Alternately one can use precision cryoembedding system to perform fresh tissue embedding. This process is undertaken so as to mount slices sections of a sample onto slides for analysis. Better than using water b c it dries more quickly and causes less freezing artifact.

Each has its own set of pros and cons. Freeze the oct containing the tissue onto the specialized metal grids that fit onto the cryostat. The three primary means of embedding tissue for sectioning are paraffin wax optimal cutting temperature oct and resin.

Store frozen blocks at 80 ºc. I put oct on the tissue before doing the touch prep rinse it off with acetone ethanol wipe let it dry then do the touch prep or smear. Oct is viscous at room temperature and miscible with h2o but freezes into a solid support at 20 c.

Which tissue embedding medium to use really comes down to how you plan to use your embedded tissue. Transfer and orientate in fresh oct in a labeled cryomold with just enough oct to cover the tissue. The section will curl if the specimen is too cold.

Problems while grossing a specimen for frozen section. Certain soft tissues such as brain are optimally frozen in m 1 medium at 3 c. The suggested cryostat temperature is between 15 and 23 c.

From wikipedia the free encyclopedia tissue embedded within optimal cutting temperature compound oct mounted on a chuck in a cryostat and ready for section production the frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. Cut cryostat sections at 5 10 µm and mount on gelatin coated histological slides. It is used most often in oncological surgery.