Paraffin Section

Section paraffin blocks at the desired thickness usually 4 5 µm on a microtome and float on a 40 c water bath.

Paraffin section. Section the paraffin embedded tissue block in 4 10 μm thickness slides on a microtome and float in a 37 c water bath containing deionized water. This tissue is then embedded in wax before being cut on a microtome. Steps trim paraffin blocks to an optimal cutting surface including the sample with a small paraffin frame cut 3 10 µm slices 5 µm is commonly used.

Process for paraffin embedding schedule as follow. Paraffin sections are more physically stable and superior to frozen sections in maintaining tissue morphology with less damage. Allow the slides to dry overnight and store slides at room.

Make sure you have enough fixative. Float the sections onto clean glass slides and microwave at 65 c for 15 min then the tissue binds to the glass. Trim fixed tissues into appropriate size and shape and place in embedding cassettes.

Paraffin section method and technique fix tissues with 10 formalin or other fixatives for 24 48 hours at room temperature. Section the paraffin embedded tissue block in 4 10 μm thickness slides on a microtome and float in a 37 c water bath containing deionized water. Use a brush to draw the section onto the knife holder place paraffin ribbon or slice in 40 45ºc water bath with a 2nd wet brush it will expand and.