Paraffin Sectioning Tips

Mount sections onto slides.

Paraffin sectioning tips. Steps trim paraffin blocks to an optimal cutting surface including the sample with a small paraffin frame cut 3 10 µm slices 5 µm is commonly used. Allow sections to air dry for 30 minutes and then bake in 45 50 º c oven overnight. Cold wax allows thinner sections to be obtained by providing support for harder elements within the tissue specimen.

Paraffin embedded tissue section is normally sliced by a rotary microtome to give a thickness of 2 7 μm. Techniques for consistent paraffin sections. Use a brush to draw the section onto the knife holder place paraffin ribbon or slice in 40 45ºc water bath with a 2nd wet brush it will expand and.

Using warm forceps transfer tissue into mold placing cut side down as it was placed in the. Put small amount of molten paraffin in mold dispensing from paraffin reservoir. This section type can be stored at 4 for long term use.

The small amount of moisture that penetrates the block from the melting ice will also make the tissue easier to cut. All of the essential aspects of cutting paraffin sections are covered including. Open cassette to view tissue sample and choose a mold that best corresponds to the size of the tissue.

Trim paraffin blocks as necessary and cut at 3 10 um 5 um is commonly used. Microtomy and paraffin section preparation is a great training aid for new microtomists and is an excellent refresher for experienced operators. With proper treatment the section reveals clear tissue structure and exact antigen location to enable high medical value pathology researches and retrospective studies.

Trimming facing and roughing blocks.