Microtome Sectioning

Leica rotary microtomes give skilled microtomists the precision control and comfort they need to get the best from every block.

Microtome sectioning. Chill paraffin embedded tissue blocks on ice before sectioning. 4a 4c as well as the specialized microtomes used to section frozen tissues fig. Sectioning a microtome is a specialized precision cutting instrument which accurately and repeatedly slices sections from a block of embedded tissue.

The thickness of sections produced during microtomy may be between fractions of 50 100 nm in ultramicrotomy to several 100 microns. Well before you throw your microtome across the room if you can pick it up in the first place then read this article. Microtome blades proprietary coated microtome blades designed to increase longevity and enhance sectioning properties.

Products include disposable premium standard and low profile blades kinves and blade carriers. So place a leica rotary microtome on your bench and consistently get the best possible sections from any block for each patient on every day. Hard tissue such as bone and cavernous tissues such as liver or heart present special challenges when sectioning.

Microtome is an instrument with the help of which sections of tissues are cut and the process of cutting thin sections is known as microtomy. Microtomy or section cutting is the technique of making the very thin slices of tissue specimens for the microscopic examination to identify the abnormalities or atypical appearance in the tissue if present and also for the study of various components of the cells or tissues like lipids enzymes antigens or antibodies immunohistochemistry cell organelles etc. Some tissues are more difficult to section than others.

Important in science microtomes are used in microscopy allowing for the preparation of samples for observation under transmitted light or electron radiation. Microtome and blade oven. Cold wax allows thinner sections to be obtained by providing support for harder elements within the tissue specimen.

Microtomy and paraffin section preparation creating great paraffin sections using a rotary microtome takes a great deal of skill and experience. Microtomy and paraffin section preparation is a great training aid for new microtomists and is an excellent refresher for experienced operators.